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Related Concept Videos

Ion Exchange01:17

Ion Exchange

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Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
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Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

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Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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Dialysis01:15

Dialysis

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Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
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Analyte Adsorption and Distribution

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In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
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Cyclable Variable Path Length Multilevel Structures for Lossless Ion Manipulations (SLIM) Platform for Enhanced Ion

Adam P Huntley1, Adam L Hollerbach1, Randolph V Norheim1

  • 1Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99354, United States.

Analytical Chemistry
|February 9, 2024
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Summary

A new multilevel Structures for Lossless Ion Manipulations (SLIM) platform enhances ion mobility-mass spectrometry (IMS-MS) resolution. This improved SLIM technology offers tunable resolving power and better ion transmission for complex sample analysis.

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Area of Science:

  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Ion mobility-mass spectrometry (IMS-MS) is crucial for analyzing complex samples and elucidating molecular structures.
  • Increasing sample complexity necessitates higher resolution IMS-MS instruments for improved molecular identification rates.

Purpose of the Study:

  • To evaluate a novel cyclable, variable path length multilevel Structures for Lossless Ion Manipulations (SLIM) platform for enhanced IMS-MS resolving power.
  • To assess improvements in ion transmission and separation quality compared to previous SLIM generations.

Main Methods:

  • Utilized an eight-level multilevel SLIM platform with a total path length of ~88 m, featuring ion escalators for ion transport.
  • Implemented an "ion cycling" mode for extended path lengths and higher IMS resolution.
  • Reduced SLIM board spacing and thickness to enhance ion transmission and reduce ion escalator residence time.

Main Results:

  • Demonstrated improved transmission for phosphazene ions, particularly low-mobility ions (e.g., ~3x improvement for m/z 2834).
  • Achieved 49% higher resolving power for GRGDS1+ ions using 4 levels compared to the previous 4-level SLIM.
  • Attained 1100 resolving power with 88 m path length and 1400 resolving power after an additional pass, validated with peptide pairs (SDGRG1+, GRGDS1+).
  • Showcased enhanced separation of complex brain total lipid extracts in both positive and negative ion modes.

Conclusions:

  • The new multilevel SLIM platform offers tunable, high resolving power suitable for a broad range of ion mobilities.
  • The enhanced SLIM design improves ion transmission, especially for low-mobility ions, advancing IMS-MS capabilities for complex sample analysis.