Related Experiment Video
Updated: Jul 4, 2025

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
Published on: May 2, 2018
Mycological Methods for Routine Air Sampling and Interpretation of Results in Operating Theaters
1Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia.
Abstract:
Many infectious diseases are transmitted via the air and are, therefore, particularly difficult to combat. These infections include various invasive mycoses caused by molds. The usual route of infection is the inhalation of conidia. In hospitals, infection can also occur through the deposition of conidia in otherwise sterile anatomical sites during surgical and other invasive procedures. Therefore, knowledge of airborne mold concentrations can lead to measures to protect patients from fungal infections. The literature on this topic contains insufficient and sometimes ambiguous information. This is evidenced by the fact that there are no international recommendations or guidelines defining the methodology of air sampling and the interpretation of the results obtained. Surgical departments, intensive care units and medical mycology laboratories are, therefore, left to their own devices, leading to significant differences in the implementation of mycological surveillance in hospitals. The aim of this mini-review is to provide an overview of the current methods of air sampling and interpretation of results used in medical mycology laboratories.
Insights
Airborne mold monitoring is crucial for preventing invasive fungal infections in hospitals. This review clarifies current air sampling methods and result interpretation for medical mycology labs.
Area of Science:
- Medical Mycology
- Infectious Disease Control
- Environmental Health
Background:
- Airborne infectious diseases, including invasive mycoses from molds, pose significant challenges.
- Inhalation of fungal conidia is a primary infection route, with hospital-acquired infections occurring during procedures.
- Current literature lacks standardized methods for air sampling and result interpretation, leading to inconsistent hospital surveillance.
Purpose of the Study:
- To provide an overview of current air sampling methods in medical mycology.
- To discuss the interpretation of results from airborne mold monitoring.
- To address the need for standardized guidelines in hospital mycological surveillance.
Main Methods:
- Review of existing literature on air sampling techniques for molds.
- Analysis of current practices in medical mycology laboratories.
- Discussion of challenges in interpreting airborne fungal data.
Main Results:
- Significant variability exists in air sampling methodologies and data interpretation across hospitals.
- Lack of international guidelines hinders consistent and effective mycological surveillance.
- Current methods require refinement to ensure patient safety and infection prevention.
Conclusions:
- Standardized protocols for airborne mold sampling and analysis are urgently needed.
- Clearer guidelines for interpreting results will improve patient protection strategies.
- Enhanced mycological surveillance is essential for combating hospital-acquired fungal infections.
More Related Videos
Related Concept Videos
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Sputum Studies II: Culture and Sensitivity
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...

