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Defense Regulatory Network Associated with circRNA in Rice in Response to Brown Planthopper Infestation.

Hou-Hong Yang1, Ya-Xuan Wang1, Jing Xiao1

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Circular RNAs (circRNAs) are involved in rice defense against the brown planthopper (BPH). This study identified differentially expressed circRNAs and their targets, revealing their role in plant-insect interactions.

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IR56 riceNilaparvata lugenscircular RNAs (circRNAs)rice-BPH interaction

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Area of Science:

  • Plant molecular biology
  • Genomics
  • Entomology

Background:

  • The brown planthopper (BPH) is a major rice pest, and existing resistance mechanisms are failing due to virulent populations.
  • Circular RNAs (circRNAs) are implicated in plant-environment interactions, but their role in insect resistance is not well understood.

Purpose of the Study:

  • To conduct a genome-wide analysis of rice circRNAs in response to BPH infestation.
  • To elucidate the mechanisms of circRNA-mediated insect resistance in rice.

Main Methods:

  • High-throughput sequencing was used to identify circRNAs in rice (IR56) under different BPH virulence groups (IR-IR56-BPH, IR-TN1-BPH) and a control (IR-CK).
  • Differential expression analysis, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed.
  • Validation of selected circRNAs, their regulatory impact on microRNAs (miRNAs) and messenger RNAs (mRNAs) was assessed.

Main Results:

  • A total of 186 circRNAs were identified, with significant numbers upregulated and downregulated in response to BPH infestation.
  • Enrichment analysis indicated that targets of differentially expressed circRNAs are involved in BPH response pathways.
  • A conceptual model was proposed where circRNAs, via parental genes and competing endogenous RNA (ceRNA) networks, participate in rice defense.

Conclusions:

  • Rice circRNAs play a significant role in the plant's defense regulatory network against BPH.
  • The findings provide insights into the molecular mechanisms underlying rice-BPH interactions and potential targets for resistance breeding.