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Calmodulin-Domain Protein Kinase PiCDPK1 Interacts with the 14-3-3-like Protein NtGF14 to Modulate Pollen Tube Growth
Nolan Scheible1, Paige M Henning1, Andrew G McCubbin1
1School of Biological Sciences, Washington State University, Pullman, WA 99164, USA.
Plants (Basel, Switzerland)
|February 10, 2024
Summary
Researchers identified new proteins interacting with PiCDPK1, a kinase crucial for pollen tube growth. One interaction with NtGF14 suggests a novel pathway regulating pollen tube extension and calcium signaling.
Area of Science:
- Plant Biology
- Molecular Cell Biology
- Biochemistry
Background:
- Calcium-mediated signaling is vital for plant pollen tube polar growth.
- PiCDPK1, a calcium-dependent protein kinase, interacts with PiRhoGDI1, influencing this growth.
Purpose of the Study:
- To identify novel substrates and interaction partners of PiCDPK1.
- To investigate the in planta interactions of putative PiCDPK1 substrates.
Main Methods:
- Utilized a pull-down assay to identify proteins interacting with PiCDPK1.
- Investigated in planta interactions of candidate proteins NtGF14 and NtREN1 through overexpression studies.
Main Results:
- Identified 123 putative PiCDPK1 interactors.
- NtGF14 (14-3-3-like protein) partially rescued PiCDPK1 overexpression phenotype, suggesting interaction.
- NtREN1 (GTPase activating protein) overexpression inhibited germination, with no significant change upon co-expression with PiCDPK1.
Conclusions:
- A novel in vivo interaction between NtGF14 and PiCDPK1 is suggested.
- Direct interaction between PiCDPK1 and NtREN1 is not supported by these findings.
- Results support the intersection of PiCDPK1 and RopGTPase pathways in pollen tube tip growth control.
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