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  11. African Under-utilized Medicinal Leafy Vegetables Studied By Microtiter Plate Assays And High-performance Thin-layer Chromatography-planar Assays

African Under-Utilized Medicinal Leafy Vegetables Studied by Microtiter Plate Assays and High-Performance Thin-Layer Chromatography-Planar Assays

Ibukun O Oresanya1, Ilkay Erdogan Orhan1, Julia Heil2

  • 1Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Emniyet, Taç Sokağı No. 3, Yenimahalle, Ankara 06330, Turkey.

Molecules (Basel, Switzerland)
|February 10, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

African leafy vegetables like Crassocephalum rubens, Solanecio biafrae, and Solanum macrocarpon show significant antioxidant and enzyme inhibitory activities. These findings support their traditional medicinal uses and highlight their potential as sources of natural bioactive compounds.

Area of Science:

  • Ethnobotany and Phytochemistry
  • Pharmacology and Natural Product Chemistry

Background:

  • Six under-utilized African leafy vegetables possess significant biological activities.
  • Traditional knowledge suggests medicinal properties for these plants.

Purpose of the Study:

  • To investigate the biological activities of six African leafy vegetables.
  • To determine total phenolic content and enzyme inhibitory effects.
  • To validate ethnomedicinal uses through scientific analysis.

Main Methods:

  • Extraction and fractionation of plant materials.
  • In vitro antioxidant assays (FRAP, CUPRAC, DPPH).
  • Enzyme inhibition assays (acetylcholinesterase, butyrylcholinesterase, tyrosinase).
  • High-performance thin-layer chromatography-multi-imaging-effect-directed analysis.
Keywords:
anticholinesteraseantidiabeticantimicrobialantioxidant

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Main Results:

  • Crassocephalum rubens, Solanecio biafrae, and Solanum macrocarpon exhibited highest total phenolic content and antioxidant activity.
  • Polar fractions of S. biafrae, C. rubens, and L. taraxacifolia showed potent acetyl- and butyrylcholinesterase inhibition.
  • C. rubens and S. biafrae fractions demonstrated significant tyrosinase inhibition.

Conclusions:

  • The study validates the ethnomedicinal uses of these African leafy vegetables.
  • Crassocephalum rubens, Solanecio biafrae, and Solanum macrocarpon are promising sources of bioactive compounds.
  • Identified compounds possess antioxidant, antidiabetic, anticholinesterase, antimicrobial, cytotoxic, and genotoxic potentials.
electrospray ionization high-resolution mass spectrometry
ethnomedicine
planar bioassays
planar chromatography