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Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots
Published on: August 16, 2020
Role of Adipose Tissue Hormones in Pathogenesis of Cryptoglandular Anal Fistula
Marcin Włodarczyk1, Jakub Włodarczyk1,2, Kasper Maryńczak1
1Department of General and Oncological Surgery, Faculty of Medicine, Medical University of Lodz, Pomorska 251, 92-213 Lodz, Poland.
Hormones from adipose tissue, including leptin and resistin, are linked to complex perianal fistulas. Reduced adiponectin and altered epithelial-to-mesenchymal transition (EMT) factors are observed in these challenging cases.
Area of Science:
- Gastroenterology and Surgical Research
- Cell Biology and Molecular Medicine
Background:
- Cryptoglandular perianal fistulas are common anorectal disorders often challenging to treat due to unclear pathogenesis.
- These conditions significantly impact patient quality of life, necessitating improved therapeutic strategies.
Purpose of the Study:
- To investigate the role of adipose tissue hormones and epithelial-to-mesenchymal transition (EMT) factors in the development of simple versus complex perianal fistulas.
- To identify potential biomarkers for classifying fistula complexity and guiding treatment.
Main Methods:
- Analysis of tissue samples from 30 simple and 30 complex perianal fistulas.
- Quantification of leptin, resistin, adiponectin, E-cadherin, MMP2, and MMP9 expression levels.
Main Results:
- Complex fistulas showed significantly elevated levels of leptin, resistin, MMP2, and MMP9 compared to simple fistulas.
- Adiponectin and E-cadherin levels were significantly lower in complex fistulas.
- Correlations observed between leptin/E-cadherin and resistin/MMP2, and adiponectin/E-cadherin.
Conclusions:
- Increased pro-inflammatory adipokines (resistin, leptin) and decreased anti-inflammatory adiponectin in complex fistulas may disrupt EMT.
- Altered expression of EMT factors (E-cadherin, MMP2, MMP9) correlates with fistula complexity.
- Findings support further research into mesenchymal stem cells for treating cryptoglandular perianal fistulas.
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