Effect of Selected Antioxidants on the In Vitro Aging of Human Fibroblasts
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View abstract on PubMed
Summary
This summary is machine-generated.Certain antioxidants, like TEMPOL, ergothioneine, and Trolox, can extend fibroblast replicative lifespan and reduce skin aging markers. Protecting mitochondrial function is key to maintaining fibroblast capacity.
Area Of Science
- Cell Biology
- Dermatology
- Biochemistry
Background
- Fibroblast replicative lifespan (RLS) is crucial for skin aging.
- Understanding factors that modify RLS can inform anti-aging strategies.
- Antioxidants are investigated for their potential to mitigate cellular senescence.
Purpose Of The Study
- To investigate the effect of six antioxidants on human dermal fibroblast RLS.
- To determine if antioxidants can influence cellular aging markers and mitochondrial function.
- To identify specific antioxidants that promote fibroblast longevity.
Main Methods
- Human dermal fibroblasts were treated with six antioxidants at 1 μM.
- Replicative lifespan (population doublings) was measured.
- Levels of reactive oxygen species (ROS), glutathione, protein carbonylation, and mitochondrial membrane potential were assessed.
Main Results
- TEMPOL, ergothioneine, and Trolox significantly extended fibroblast RLS.
- These antioxidants also lowered p21 expression at later passages.
- Ergothioneine and resveratrol reduced protein carbonylation, while TEMPOL and coumaric acid decreased glutathione.
Conclusions
- Antioxidants like TEMPOL, ergothioneine, and Trolox can prolong fibroblast RLS, suggesting a role in anti-aging.
- Elevated mitochondrial membrane potential correlates with extended RLS, highlighting mitochondria's importance.
- Specific antioxidant mechanisms, including ROS modulation and protection against oxidative damage, contribute to fibroblast longevity.
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