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  11. A Multi-omics Approach Reveals Enrichment In Metabolites Involved In The Regulation Of The Glutathione Pathway In Lin28b-dependent Cancer Cells.

A Multi-Omics Approach Reveals Enrichment in Metabolites Involved in the Regulation of the Glutathione Pathway in LIN28B-Dependent Cancer Cells.

Matteo Stocchero1,2, Diana Corallo3, Silvia Bresolin1

  • 1Department of Women and Children's Health, University of Padova, 35128 Padova, Italy.

International Journal of Molecular Sciences
|February 10, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

High LIN28B levels in neuroblastoma rewire cell metabolism, increasing specific metabolite release and decreasing glucose uptake. This metabolic shift may explain adverse outcomes and chemoresistance in high-risk patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Metabolomics

Background:

  • LIN28B is an RNA-binding protein and a risk factor in high-risk neuroblastoma.
  • LIN28B is associated with poor treatment outcomes, metastasis, and chemoresistance.
  • The metabolic impact of LIN28B in neuroblastoma is currently unknown.

Purpose of the Study:

  • To investigate the global metabolic program influenced by LIN28B expression in neuroblastoma.
  • To understand the time-dependent metabolic rewiring induced by LIN28B.

Main Methods:

  • Multi-omics approach integrating transcriptome and metabolome data.
  • Analysis of metabolic changes in neuroblastoma cells with varying LIN28B expression levels over time.

Main Results:

Keywords:
LIN28Bglutathione metabolismmetabolomeneuroblastoma

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  • LIN28B expression significantly rewires neuroblastoma cell metabolism.
  • Increased release of metabolites from glutathione and aminoacyl-tRNA pathways observed.
  • Reduced glucose uptake in cells with escalating LIN28B expression.

Conclusions:

  • LIN28B plays a critical role in regulating neuroblastoma cell metabolism.
  • LIN28B may disrupt the redox balance in neuroblastoma cells.
  • Findings provide insights into LIN28B-associated adverse outcomes and potential therapeutic targets.
omics integration
transcriptome