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Published on: September 27, 2015
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Y-Box-Binding Proteins Have a Dual Impact on Cellular Translation
Irina A Eliseeva1, Andrey I Buyan1, Egor A Smolin1
1Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Russia.
International Journal of Molecular Sciences
|February 10, 2024
Summary
Y-box-binding proteins (YB proteins) like YB-1 and YB-3 can be interchanged. While stimulating global translation, these YB proteins specifically inhibit the translation of their direct mRNA targets, suggesting functional redundancy.
Area of Science:
- Molecular Biology
- Gene Regulation
Background:
- Y-box-binding proteins (YB proteins) are crucial for gene expression regulation.
- YB-1 and YB-3 share structural similarities, hinting at potential functional overlap.
Purpose of the Study:
- To investigate the functional interchangeability of somatic YB proteins YB-1 and YB-3.
- To determine the impact of YB-1 and YB-3 on mRNA abundance and translation.
Main Methods:
- RNA sequencing (RNA-seq) to analyze mRNA abundance.
- Ribosome profiling (Ribo-seq) to assess mRNA translation.
- CLIP-identified mRNA targets were analyzed in HEK293T cells expressing YB-1, YB-3, or neither.
Main Results:
- YB proteins exhibit a dual role in translation: stimulating global translation while inhibiting the translation of their direct mRNA targets.
- YB-1 and YB-3 demonstrated similar inhibitory effects on the translation of their identified mRNA targets.
- Functional interchangeability was observed for the translational inhibition of specific mRNA targets.
Conclusions:
- YB-1 and YB-3 can functionally substitute for each other in inhibiting the translation of their direct mRNA targets.
- The findings highlight the conserved function of YB proteins in translational control.
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