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Transcriptome Analysis in Mexican Adults with Acute Lymphoblastic Leukemia
Gabriela Marisol Cruz-Miranda1,2, Irma Olarte-Carrillo3, Diego Alberto Bárcenas-López1,2
1Programa de Doctorado, Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
International Journal of Molecular Sciences
|February 10, 2024
Summary
This study identified key genes and pathways involved in adult Acute Lymphoblastic Leukemia (ALL). Findings highlight specific gene expression changes linked to patient outcomes, offering insights into this complex blood cancer.
Area of Science:
- Hematology
- Molecular Biology
- Oncology
Background:
- Acute Lymphoblastic Leukemia (ALL) in adults presents heterogeneous clinical and molecular features, challenging treatment and outcomes.
- Despite survival rate improvements, understanding the molecular basis of adult ALL remains crucial for therapeutic advancements.
Purpose of the Study:
- To identify aberrantly expressed genes in adult ALL bone marrow samples.
- To elucidate the functional roles and pathways associated with differentially expressed genes in adult ALL.
Main Methods:
- Transcriptomic analysis using Affymetrix HTA 2.0 arrays.
- Differential gene expression analysis with strict statistical cutoffs (±2-fold change, p<0.05, FDR<0.05).
- Gene Ontology, DAVID, IPA, and PPI network construction for functional and pathway enrichment.
Main Results:
- Identified 871 differentially expressed genes (DEGs) in adult ALL.
- Top upregulated genes include DNTT, MYB, EBF1, SOX4, ERG; top downregulated genes include PTGS2, PPBP, ADGRE3, LUCAT1, VCAN.
- ERG, CDK6, and SOX4 expression correlated with relapse and mortality risk.
- Altered biological processes include immune system regulation, cellular response to stimulus, and apoptosis signaling.
Conclusions:
- Transcriptome analysis reveals a distinct set of genes associated with adult ALL.
- These findings underscore the relevance of identified genes in the development and progression of adult ALL.
- The study provides a molecular basis for understanding adult ALL heterogeneity and potential therapeutic targets.
