Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

4.9K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
4.9K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

13.9K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
13.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunotherapy for early-stage cutaneous squamous cell carcinoma.

Journal for immunotherapy of cancer·2026
Same author

A high-penetrance intergenic variant at 9p21 confers melanoma susceptibility.

Research square·2026
Same author

Discontinuation of combo immunotherapy and outcome of patients with melanoma brain metastases.

Journal for immunotherapy of cancer·2026
Same author

Dissecting the Spectrum of Rare BRAF Mutations in Melanoma: A Nation-Wide Study by the Italian Melanoma Intergroup (IMI).

Pigment cell & melanoma research·2026
Same author

End‑of‑life care in advanced melanoma: a retrospective cohort study from an Italian comprehensive cancer center.

BMC cancer·2026
Same author

High BRAF variant allele frequency predicts poor outcomes in metastatic melanoma patients treated with BRAF/MEK inhibitors.

Journal of translational medicine·2025
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Jul 3, 2025

Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography
05:46

Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography

Published on: December 2, 2022

1.7K

Machine Learning Methods for Gene Selection in Uveal Melanoma.

Francesco Reggiani1, Zeinab El Rashed1, Mariangela Petito1,2

  • 1Laboratory of Gene Expression Regulation, IRCCS Ospedale Policlinico San Martino, 16132 Genova, Italy.

International Journal of Molecular Sciences
|February 10, 2024
PubMed
Summary
This summary is machine-generated.

This study compares bioinformatics strategies to identify uveal melanoma (UM) genes linked to metastasis. It validates findings using multi-gene scores, aiming to improve treatment targets for this rare eye cancer.

Keywords:
data fusionmulti-domain datauveal melanoma

More Related Videos

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances
07:35

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances

Published on: October 11, 2018

7.5K
A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
07:41

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

2.4K

Related Experiment Videos

Last Updated: Jul 3, 2025

Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography
05:46

Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography

Published on: December 2, 2022

1.7K
Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances
07:35

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances

Published on: October 11, 2018

7.5K
A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
07:41

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

2.4K

Area of Science:

  • Oncology
  • Bioinformatics
  • Genomics

Background:

  • Uveal melanoma (UM) is the most common primary intraocular malignancy.
  • Metastatic UM has limited therapeutic options and poor survival rates.
  • Genomic studies have deepened tumor biology understanding but not improved outcomes.

Purpose of the Study:

  • To compare different bioinformatics strategies for detecting relevant genes in metastatic uveal melanoma.
  • To identify potential molecular targets for novel therapies.
  • To validate detected targets using multi-gene score analysis.

Main Methods:

  • Utilized next-generation sequencing (NGS) data from The Cancer Genome Atlas (TCGA) uveal melanoma (UVM) dataset.
  • Compared single-domain (e.g., DEG analysis, deep learning) and data-fusion approaches.
  • Validated candidate genes using multi-gene score analysis on a separate UM microarray dataset.

Main Results:

  • Identified and compared various gene detection strategies for metastatic UM prediction.
  • Validated potential therapeutic targets through multi-gene score analysis.
  • Demonstrated the utility of integrated bioinformatics approaches for target discovery.

Conclusions:

  • Bioinformatics analysis of NGS data can reveal key genes associated with uveal melanoma metastasis.
  • Data integration methods show promise in identifying robust molecular targets.
  • Further validation is crucial for translating these findings into clinical applications.