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Updated: Sep 29, 2026

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
Published on: September 1, 2009
[Cytogenetics of human gametes: its application to the study of fertilization anomalies]
Abstract:
A study of 100 sperm karyotypes from 8 normal subjects was carried out using the technique of in vitro hetero-specific human-hamster fertilisation. 19 of those showed some abnormality. 17% of those were hyperploidies or hypoploidies and 2% showed structural abnormalities. A study carried out at the same time on 34 hamster egg karyotypes showed 6 hypoploidies and 1 hyperploidy. In effect oa cytogenetic technique which is very similar to this one makes it possible to analyse the human oocyte karyotype. The results found in these samples of motile sperms show a higher incidence of abnormality than in other published series, but these figures can not be extrapolated to the composition of in vivo whole sperms.
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