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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jul 3, 2025

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Published on: August 9, 2022

Progress on fluorescent RNA and fluorescent NA-based biosensing technology.

Fang-Ting Zuo^1,2,3, Ya-Qiang Zhang^1,2,3, Hui-Min Yang^1,2

¹Interdisciplinary Research Center of Optogenetics and Synthetic Biology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.

Yi Chuan = Hereditas

|February 10, 2024

Summary

This summary is machine-generated.

Genetically encoded fluorescent RNA enables real-time RNA imaging and biosensing in live cells. This review explores its development, applications in life sciences, and future potential for biomedical sensing.

Keywords:

RNA imaging biosensing fluorescent RNA fluorescent labeling

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Area of Science:

Molecular Biology
Biotechnology
Cellular Imaging

Background:

Fluorescent RNA is an emerging technique for in situ RNA labeling and imaging in live cells.
Understanding RNA function and regulation is crucial in molecular biology.
RNA imaging and biosensing are vital for life science research and biomedical development.

Approach:

This review introduces genetically encoded fluorescent RNA development.
It details applications in live-cell RNA imaging and biosensing.
The review discusses future directions and challenges in fluorescent RNA technology.

Key Points:

Genetically encoded fluorescent RNA allows for precise RNA tracking within live cells.
Fluorescent RNA biosensors enable dynamic detection of metabolites and proteins.
This technology provides valuable tools for basic research and biomedical applications.

Conclusions:

Fluorescent RNA technology is rapidly advancing, offering new possibilities for biological research.
Further development is needed to overcome current challenges and expand applications.
This field holds significant promise for future innovations in live-cell imaging and biosensing.