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Progress on the quality control technology of next generation sequencing library.
Meng-Nan Cui1, Yan Guo1, Ya-Rong Wu1
1State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Yi Chuan = Hereditas
|February 10, 2024
Summary
High-throughput sequencing library construction quality control is vital for reliable results. This review details techniques to improve accuracy and efficiency in life sciences and medicine.
Area of Science:
- Life Sciences
- Medicine
- Genomics
Background:
- High-throughput sequencing (HTS) is a cornerstone technology in life sciences and medicine.
- The HTS workflow involves nucleic acid extraction, library construction, sequencing, and data analysis.
- Library construction is a critical step influencing downstream data quality and reliability.
Conclusions:
- Effective quality control in library construction is essential for enhancing HTS data reliability and reducing errors.
- Informed selection of QC techniques can significantly improve the efficiency and accuracy of sequencing projects.
- This review serves as a foundational reference for researchers and professionals utilizing HTS technologies.
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