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FTIR microspectroscopic study of gastric cancer AGS cells apoptosis induced by As2O3

Chao Li1, Jie Shi2, Yongan Wang2

  • 1National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui 230029, China; School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, China; The Second Affiliated Hospital, Anhui Medical University, Hefei, Anhui 230601, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|February 10, 2024
PubMed

Insights

Arsenic trioxide (As2O3) induces apoptosis in gastric cancer cells. Fourier transform infrared (FTIR) microspectroscopy reveals significant biomacromolecular changes, offering a new method for apoptosis research.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Spectroscopy

Background:

  • Arsenic trioxide (As2O3) exhibits anti-gastric cancer properties, but its underlying mechanisms remain largely unknown.
  • Understanding the molecular alterations during As2O3-induced apoptosis is crucial for developing targeted cancer therapies.

Purpose of the Study:

  • To investigate the biomacromolecular changes in human gastric cancer AGS cells induced by As2O3.
  • To explore the potential of Fourier transform infrared (FTIR) microspectroscopy in analyzing As2O3-induced apoptosis.

Main Methods:

  • Human gastric cancer AGS cells were treated with As2O3.
  • Flow cytometry was employed to confirm apoptosis induction.
  • Fourier transform infrared (FTIR) microspectroscopy was utilized to detect biomacromolecular changes.
  • Spectral data were analyzed using peak-area ratios, Principal Component Analysis (PCA), and curve fitting.

Main Results:

  • Flow cytometry confirmed that As2O3 induced apoptosis in AGS cells.
  • FTIR microspectroscopy detected significant alterations in lipid content and the structure of proteins and DNA.
  • Analysis revealed changes in lipid and DNA content and protein structure, with a linear correlation between spectral ratios and apoptosis rate.
  • PCA and curve fitting indicated significant changes in nucleic acids and proteins.

Conclusions:

  • As2O3 effectively induces apoptosis in gastric cancer cells through specific biomacromolecular alterations.
  • FTIR microspectroscopy is a sensitive technique for monitoring these biomacromolecular changes during apoptosis.
  • This study highlights FTIR microspectroscopy as a valuable tool for investigating apoptosis in cancer research.
Keywords:
ApoptosisAs(2)O(3)Biomacromolecular changesFTIR microspectroscopyGastric cancer

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