Related Experiment Video
Updated: May 7, 2026
Competitive Genomic Screens of Barcoded Yeast Libraries
Published on: August 11, 2011
mBARq: a versatile and user-friendly framework for the analysis of DNA barcodes from transposon insertion libraries,
Anna Sintsova1,2, Hans-Joachim Ruscheweyh1,2, Christopher M Field1,2
1Department of Biology, Institute of Microbiology, ETH Zurich, Zurich 8093, Switzerland.
Motivation:
DNA barcoding has become a powerful tool for assessing the fitness of strains in a variety of studies, including random transposon mutagenesis screens, attenuation of site-directed mutants, and population dynamics of isogenic strain pools. However, the statistical analysis, visualization, and contextualization of the data resulting from such experiments can be complex and require bioinformatic skills.
Results:
Here, we developed mBARq, a user-friendly tool designed to simplify these steps for diverse experimental setups. The tool is seamlessly integrated with an intuitive web app for interactive data exploration via the STRING and KEGG databases to accelerate scientific discovery.
Availability And Implementation:
The tool is implemented in Python. The source code is freely available (https://github.com/MicrobiologyETHZ/mbarq) and the web app can be accessed at: https://microbiomics.io/tools/mbarq-app.
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