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RNA-binding protein IGF2BP2 suppresses metastasis of clear cell renal cell carcinoma by enhancing CKB mRNA stability

Junwu Ren1, Bo Huang2, Wei Li3

  • 1College of Pharmacy, Chongqing Medical University, Chongqing 400016, PR China.

Translational Oncology
|February 11, 2024
PubMed

Insights

Insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) suppresses clear cell renal cell carcinoma (ccRCC) metastasis. This RNA binding protein enhances Creatine Kinase B (CKB) expression, offering a potential therapeutic target for kidney cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Clear cell renal cell carcinoma (ccRCC) is an aggressive kidney cancer with poor prognosis.
  • RNA binding proteins (RBPs) are critical in gene regulation and implicated in cancer development.
  • RBPs represent potential therapeutic targets for ccRCC.

Purpose of the Study:

  • To identify and validate novel RNA binding proteins (RBPs) as therapeutic targets for ccRCC.
  • To investigate the role of insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) in ccRCC progression.
  • To elucidate the molecular mechanism by which IGF2BP2 affects ccRCC metastasis.

Main Methods:

  • Screening and validation of RBPs in ccRCC tissues and cell lines.
  • In vitro and in vivo functional assays to assess ccRCC cell migration and invasion.
  • RIP-seq and actinomycin D experiments to determine mRNA targets and stability.
  • Analysis of IGF2BP2 and Creatine Kinase B (CKB) expression levels.

Main Results:

  • IGF2BP2 was found to be downregulated in ccRCC tissues and cell lines.
  • IGF2BP2 significantly suppressed ccRCC cell migration and invasion both in vitro and in vivo.
  • IGF2BP2 enhances Creatine Kinase B (CKB) expression by increasing CKB mRNA stability.
  • RIP-seq identified CKB mRNA as a target of IGF2BP2.

Conclusions:

  • IGF2BP2 acts as a metastasis suppressor in ccRCC.
  • IGF2BP2 inhibits ccRCC metastasis by enhancing CKB expression.
  • IGF2BP2 is a potential therapeutic target for ccRCC treatment.
Keywords:
CKBClear cell renal cell carcinomaIGF2BP2RNA binding proteinmRNA stability

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