Chemoenzymatic synthesis and immunological evaluation of sialyl-Thomsen-Friedenreich (sTF) antigen conjugate to CRM197
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.The sialyl-Thomsen-Friedenreich (sTF) antigen, a tumor marker, was successfully prepared and conjugated to CRM197. This sTF-CRM197 conjugate generated specific antibodies that target cancer cells and induce cell death.
Area Of Science
- Immunology
- Oncology
- Carbohydrate Chemistry
Background
- Sialyl-Thomsen-Friedenreich (sTF) is a tumor-associated carbohydrate antigen (TACA) overexpressed in many human cancers.
- sTF presents a potential target for cancer vaccine development due to its specific expression on malignant cells.
Purpose Of The Study
- To synthesize and characterize an sTF-CRM197 conjugate for potential cancer vaccine applications.
- To evaluate the immunogenicity and efficacy of the sTF-CRM197 conjugate in preclinical models.
Main Methods
- Chemical and enzymatic synthesis of the sTF antigen.
- Covalent conjugation of the sTF antigen to the carrier protein CRM197.
- Immunization of BALB/c mice with the sTF-CRM197 conjugate.
- Serological analysis, FACS, and complement-dependent cytotoxicity (CDC) assays.
Main Results
- The sTF-CRM197 conjugate successfully elicited high titers of specific IgG antibodies in mice.
- Generated antisera specifically recognized and bound to sTF-positive cancer cells (T-47D) in FACS experiments.
- The conjugate demonstrated effective antibody-mediated complement-dependent cytotoxicity (CDC) against cancer cells.
Conclusions
- The sTF-CRM197 conjugate is immunogenic and generates specific antibodies capable of targeting sTF-expressing cancer cells.
- The conjugate shows potential as a basis for developing a cancer vaccine targeting sTF.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
