Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decoding TCR specificity and T cell fate at the immunological synapse.

The Journal of experimental medicine·2026
Same author

Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancer.

Nature communications·2026
Same author

Extracellular CD44 lactylation impairs CD8<sup>+</sup> T cell function in KRAS-mutant colorectal cancer.

Nature metabolism·2026
Same author

The ubiquitin ligase KLHL6 drives resistance to CD8<sup>+</sup> T cell dysfunction.

Nature·2026
Same author

Dual gains from targeting MondoA in T cells.

Nature metabolism·2025
Same author

Bi-directional metabolic reprogramming between cancer cells and T cells reshapes the anti-tumor immune response.

PLoS biology·2025

Related Experiment Video

Updated: Jul 3, 2025

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
00:09

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

10.7K

Deciphering Membrane-Protein Interactions and High-Throughput Antigen Identification with Cell Doublets.

Yuqian Wang1,2, Zhe Wang1,2, Juan Yang1,2

  • 1National Key Laboratory of Immunity and Inflammation, Suzhou Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, Jiangsu, 215123, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|February 11, 2024
PubMed
Summary

Researchers discovered a new method, DoubletSeeker, to identify cell interactions by detecting cell doublets. This tool accurately identifies T cell receptor (TCR) and antigen interactions, aiding in disease research and immunotherapy development.

Keywords:
T cell antigen discoveryTCRdoubletsmembrane‐protein interactionpMHC

More Related Videos

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

7.2K
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
00:07

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

8.4K

Related Experiment Videos

Last Updated: Jul 3, 2025

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
00:09

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

10.7K
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

7.2K
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
00:07

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

8.4K

Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Understanding cellular interactions is crucial for disease mechanisms and therapeutic strategies.
  • Specific membrane ligand-receptor interactions can lead to the formation of cell doublets.

Purpose of the Study:

  • To develop a high-throughput method for identifying ligand-receptor interactions based on cell doublet formation.
  • To validate the method's ability to accurately identify T cell receptor (TCR)-antigen interactions.

Main Methods:

  • Developed DoubletSeeker, a novel high-throughput method leveraging cell doublet formation.
  • Applied DoubletSeeker to identify T cell receptor (TCR)-peptide major histocompatibility complex (pMHC) interactions.
  • Utilized a complex library-on-library screening approach.

Main Results:

  • DoubletSeeker reliably identifies ligand-receptor interactions with high sensitivity and specificity.
  • Accurately identified T cell receptor (TCR)-antigen interactions.
  • Successfully identified three mutant TCRs recognizing the MART-1 epitope.

Conclusions:

  • DoubletSeeker is a powerful tool for identifying cellular interactions and discovering novel immunotherapy targets.
  • The method advances the investigation of tumor immunology and facilitates the development of new cancer therapies.