Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A male-derived volatile sex pheromone in Caenorhabditis nematodes identified through its mimicry by a predator.

Communications biology·2026
Same author

Identification of <i>srh-30</i> as a 2-nonanone receptor in <i>C. elegans</i>.

bioRxiv : the preprint server for biology·2026
Same author

Joubert syndrome gene <i>fam149b1</i> homolog, <i>xbx-4</i> , is required for multiple sensory behaviors.

microPublication biology·2026
Same author

Whole-organism spatial transcriptomics at single-cell resolution in <i>C. elegans</i>.

bioRxiv : the preprint server for biology·2026
Same author

The forkhead transcription factor FKH-7/FOXP acts in Caenorhabditis elegans chemosensory neurons to shape a life history strategy.

Genetics·2026
Same author

ODin (Orthology Data driven Interaction) Predictor: A Client‑Side Web Application for Unveiling Biological Connections Through Orthology from Alliance of Genome Resources.

microPublication biology·2026

Related Experiment Video

Updated: Jul 3, 2025

In vivo and In vitro Rearing of Entomopathogenic Nematodes Steinernematidae and Heterorhabditidae
08:56

In vivo and In vitro Rearing of Entomopathogenic Nematodes Steinernematidae and Heterorhabditidae

Published on: September 22, 2014

20.6K

An improved solid medium-based culturing method for Steinernema hermaphroditum.

Nathan Y Rodak1,2, Chieh-Hsiang Tan1, Paul W Sternberg1

  • 1Division of Biology and Biological Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA.

Micropublication Biology
|February 12, 2024
PubMed
Summary

We developed an improved culturing method for the hermaphroditic nematode Steinernema hermaphroditum, facilitating genetic studies. This method supports rapid growth and developmental research, revealing insights into reproductive strategies.

More Related Videos

Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products
12:40

Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products

Published on: April 6, 2015

18.5K
Culturing Caenorhabditis elegans in Axenic Liquid Media and Creation of Transgenic Worms by Microparticle Bombardment
08:26

Culturing Caenorhabditis elegans in Axenic Liquid Media and Creation of Transgenic Worms by Microparticle Bombardment

Published on: August 2, 2014

17.0K

Related Experiment Videos

Last Updated: Jul 3, 2025

In vivo and In vitro Rearing of Entomopathogenic Nematodes Steinernematidae and Heterorhabditidae
08:56

In vivo and In vitro Rearing of Entomopathogenic Nematodes Steinernematidae and Heterorhabditidae

Published on: September 22, 2014

20.6K
Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products
12:40

Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products

Published on: April 6, 2015

18.5K
Culturing Caenorhabditis elegans in Axenic Liquid Media and Creation of Transgenic Worms by Microparticle Bombardment
08:26

Culturing Caenorhabditis elegans in Axenic Liquid Media and Creation of Transgenic Worms by Microparticle Bombardment

Published on: August 2, 2014

17.0K

Area of Science:

  • Nematology
  • Developmental Biology
  • Genetics

Background:

  • Steinernema hermaphroditum is a unique hermaphroditic entomopathogenic nematode.
  • It offers significant potential for genetic studies of symbiosis and other biological areas.
  • It is evolutionarily, ecologically, and morphologically distinct from commonly used lab nematodes.

Purpose of the Study:

  • To describe an improved solid medium-based culturing method for S. hermaphroditum.
  • To facilitate phenotypic analysis and genetic manipulation.
  • To support developmental studies and analyze reproductive strategies.

Main Methods:

  • Development of a novel solid medium-based culturing technique for S. hermaphroditum.
  • Observation and documentation of worm growth rates and uniformity.
  • Measurement of individual animal brood sizes.

Main Results:

  • The improved method supports rapid and uniform growth of S. hermaphroditum.
  • This uniform growth is suitable for developmental studies.
  • Brood size in S. hermaphroditum is large but follows a similar trajectory to Caenorhabditis elegans, suggesting conserved reproductive constraints.

Conclusions:

  • The new culturing method enhances the utility of S. hermaphroditum for biological research.
  • S. hermaphroditum serves as a valuable model for studying symbiosis and development.
  • Comparative reproductive studies suggest common evolutionary pressures on hermaphroditic nematodes.