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A simple, rapid, high yield isolation and purification procedure for chloroperoxidase isoenzymes
Preparative Biochemistry
|January 1, 1985
Abstract:
A simple four-step procedure has been developed for isolation of chloroperoxidase from the mold Caldariomyces fumago. Polyethyleneglycol precipitation of the contaminating pigment in the growth medium, followed by chromatography of the soluble enzyme fraction on a QAE-ZetaPrep-250 cartridge and ammonium sulfate precipitation affords isolation of the chloroperoxidase. Extensive dialysis and chromatography on DE-53 cellulose allows the separation and further purification of chloroperoxidase A and B isoenzymes.