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Related Experiment Video

Updated: Jul 3, 2025

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of

Nathan Felcher¹, Daisy Achiriloaie¹, Brian Lee²

¹W.M. Keck Science Department, Scripps College.

Journal of Visualized Experiments : Jove

|February 12, 2024

Summary

Researchers can now build their own light-sheet fluorescence microscope (LSFM) for studying cytoskeleton dynamics. This guide details constructing a single-objective light-sheet (SOLS) microscope using readily available parts for advanced soft matter research.

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Area of Science:

Soft Matter Physics
Biophysics
Microscopy

Background:

Cytoskeleton composites are key models for non-equilibrium soft matter.
Optical sectioning is crucial for capturing dynamics in 3D networks.
Light-sheet fluorescence microscopy (LSFM) offers a cost-effective alternative to confocal microscopy.

Purpose of the Study:

To provide a beginner's guide for building a versatile LSFM.
To make advanced microscopy techniques accessible to cytoskeleton researchers.
To enable custom instrument design for specific research needs.

Main Methods:

Construction of a single-objective light-sheet (SOLS) microscope from off-the-shelf components.
Detailed explanation of each component's function for user modification.
Demonstration of the SOLS instrument for biological sample visualization.

Main Results:

A functional SOLS microscope was successfully built.
The instrument allows for optical sectioning of dense 3D networks.
Visualization of asters in kinesin-driven microtubule networks was achieved.

Conclusions:

LSFM, particularly the SOLS design, is a powerful and accessible tool for cytoskeleton research.
Custom-built LSFM instruments can be tailored to specific experimental requirements.
This guide empowers researchers to adopt advanced imaging techniques for soft matter studies.