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Updated: Jul 3, 2025

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription
Published on: July 26, 2019
Transcription activation in Escherichia coli and Salmonella
Stephen J W Busby1, Douglas F Browning1,2
1School of Biosciences & Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom.
Abstract:
Promoter-specific activation of transcript initiation provides an important regulatory device in Escherichia coli and Salmonella. Here, we describe the different mechanisms that operate, focusing on how they have evolved to manage the "housekeeping" bacterial transcription machinery. Some mechanisms involve assisting the bacterial DNA-dependent RNA polymerase or replacing or remodeling one of its subunits. Others are directed to chromosomal DNA, improving promoter function, or relieving repression. We discuss how different activators work together at promoters and how the present complex network of transcription factors evolved.
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