Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ion Exchange01:17

Ion Exchange

592
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
592
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

833
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
833
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

610
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
610
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

492
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
492
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

749
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
749
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

5.2K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isolation, Identification, and Growth Promotion Effects of Plant Growth-Promoting Rhizobacteria on Alfalfa.

Microorganisms·2026
Same author

Detection of Pantoea ananatis in cerebrospinal fluid: a case report of central nervous system infection after craniotomy.

BMC infectious diseases·2026
Same author

Bayesian benchmark dose assessment of fluoride exposure and renal function impairment in adolescents.

International journal of environmental health research·2026
Same author

Magnetic Fields Enrich Paramagnetic Ion Concentrations via Magnetophoresis and Magnet-Induced Convection.

The journal of physical chemistry. B·2026
Same author

Enhancing Sensitivity in Targeted Single-Cell Proteomics by Coupling a Dual Ion Funnel Interface with Triple Quadrupole Mass Spectrometer.

Analytical chemistry·2026
Same author

Integrating transcriptomic, physiological, and biochemical studies revealing the role of endogenous ABA and GA<sub>3</sub> in the germination of quinoa seed.

Frontiers in plant science·2026
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026
Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026
Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jul 3, 2025

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions
10:22

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

Published on: June 16, 2014

18.2K

Mobility Selective Ion Soft-Landing and Characterization Enabled Using Structures for Lossless Ion Manipulation.

Jung Y Lee1, Ailin Li1, Venkateshkumar Prabhakaran2

  • 1Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.

Analytical Chemistry
|February 12, 2024
PubMed
Summary
This summary is machine-generated.

Structures for Lossless Ion Manipulation (SLIM) enables ion selection and deposition based on ion mobility, not just mass-to-charge ratio. This technique allows for targeted collection and analysis of specific ions from complex mixtures.

More Related Videos

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.3K
Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

9.2K

Related Experiment Videos

Last Updated: Jul 3, 2025

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions
10:22

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

Published on: June 16, 2014

18.2K
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.3K
Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

9.2K

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Mass Spectrometry

Background:

  • Conventional ion-soft landing relies on mass-to-charge (m/z) ratio for molecular selection.
  • Limitations exist in achieving precise molecular selection using m/z alone in complex mixtures.
  • Structures for Lossless Ion Manipulation (SLIM) offers advanced ion manipulation capabilities.

Purpose of the Study:

  • To demonstrate the utility of SLIM for mobility-based ion selection and deposition.
  • To showcase SLIM's capability to select and deposit specific ions from mixtures.
  • To characterize deposited ions using subsequent analytical techniques.

Main Methods:

  • Utilized SLIM's dynamic rerouting for mobility-based ion selection.
  • Rerouted selected ion mobilities to a deposition surface.
  • Analyzed deposited ions using SLIM-qToF, TEM, and EDS spectroscopy.

Main Results:

  • Successfully selected and deposited a specific mobility range of phosphazene ions.
  • Collected and analyzed selected tetra-alkyl ammonium (TAA) ions (C6, C7), confirming their identity via IMS/MS.
  • Characterized triply charged tungsten-polyoxometalate anions (WPOM), deposited selected ions, and confirmed identity using TEM and EDS.

Conclusions:

  • SLIM technology provides a novel platform for mobility-based ion selection and deposition.
  • This method enables the targeted isolation and analysis of specific molecular species from complex samples.
  • The study presents theoretical insights into ion landing energies and deposition characteristics.