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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Published on: August 9, 2011

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Molecular Characteristics and Virulence Profile of Clinical Listeria monocytogenes Isolates in Northern Taiwan,

Tsui-Ping Liu1,2, Lee-Chung Lin3, Shih-Cheng Chang4

  • 1Infectious Control Office, Tao-Yuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan.

Foodborne Pathogens and Disease
|February 12, 2024
PubMed

Insights

This study tracked Listeria monocytogenes in Taiwan, finding specific strains with virulence genes. Continued surveillance is crucial for preventing foodborne illness outbreaks.

Area of Science:

  • Foodborne Pathogen Research
  • Molecular Epidemiology
  • Public Health Microbiology

Background:

  • Listeria monocytogenes is a significant foodborne pathogen causing severe illness and high mortality.
  • Limited data exists on L. monocytogenes prevalence and characteristics in Taiwan.
  • Understanding molecular epidemiology is vital for controlling foodborne diseases.

Purpose of the Study:

  • To conduct molecular epidemiological surveillance of human clinical L. monocytogenes isolates in northern Taiwan.
  • To analyze the distribution of virulence genes, specifically Listeria pathogenicity islands (LIPIs), within these isolates.
  • To identify potentially hypervirulent strains and inform public health strategies.

Main Methods:

  • Analysis of 176 human clinical L. monocytogenes isolates collected from 2009-2019.
  • Serogrouping, Multilocus Sequence Typing (MLST) to determine sequence types (STs) and clonal complexes (CCs).
  • Detection and distribution analysis of four Listeria pathogenicity islands (LIPI-1, -2, -3, -4).

Main Results:

  • Isolates predominantly belonged to serogroups IIa (46%) and IIb (40.3%).
  • Eighteen sequence types (STs) and 13 clonal complexes (CCs) were identified, with six major STs comprising 84% of isolates.
  • LIPI-1 and LIPI-2 were ubiquitous; LIPI-3 and LIPI-4 were found in specific STs/CCs, with 39.1% of isolates potentially hypervirulent due to LIPI-3/LIPI-4 presence.

Conclusions:

  • Dominant L. monocytogenes serogroups and sequence types in Taiwan were identified.
  • The presence of LIPI-3 and LIPI-4 indicates a significant proportion of potentially hypervirulent strains.
  • Ongoing molecular surveillance of Listeria in food is essential for preventing foodborne disease.
Keywords:
Listeria monocytogenesListeria pathogenicity islandsmultilocus sequence typing (MLST)serogroups

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