A green strategy for collagen extraction from tannery raw trimmings using papain enzyme: Process optimization by MW-TOPSIS for enhanced yield

Mysha Maliha ¹, Taslim Ur Rashid ², Mohammed Mizanur Rahman ³

⁰Institute of Leather Engineering and Technology, University of Dhaka, Dhaka 1209, Bangladesh.

International Journal of Biological Macromolecules +

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February 12, 2024

View abstract on PubMed

Summary

This study presents a green method for extracting collagen from leather industry waste using papaya enzymes. This enzymatic hydrolysis achieved a higher yield (91%) than traditional acid methods, offering a sustainable bioresource recovery solution.

Area Of Science

Biotechnology
Green Chemistry
Materials Science

Background

Leather industry generates significant protein-rich trimming waste.
Sustainable utilization of this waste is crucial for environmental and economic benefits.
Collagen recovery from waste offers a pathway to valuable bioresource transformation.

Purpose Of The Study

To develop an eco-friendly and cost-effective method for collagen extraction from tannery waste.
To optimize enzymatic hydrolysis using papain from papaya leaves.
To compare the efficiency of enzymatic hydrolysis with conventional methods.

Main Methods

Optimization of enzymatic hydrolysis parameters (enzyme concentration, temperature, time, pH).
Collagen extraction using papain enzyme from papaya leaf powder.
Comparison with acetic acid extraction and evaluation using an integrated MW-TOPSIS framework.

Main Results

Optimized papain enzyme hydrolysis yielded 91% collagen recovery.
Acetic acid method yielded 84% collagen recovery.
Enzymatic hydrolysis demonstrated superior performance with the highest closeness coefficient (Ri=0.40) in the MW-TOPSIS framework.

Conclusions

Papaya enzyme-based hydrolysis is a superior, green alternative for collagen extraction from tannery waste.
This method offers a sustainable approach to valorize protein-rich industrial byproducts.
The findings support the transformation of waste into valuable biomaterials.

Keywords:

Enzymatic hydrolysis Leather trimming waste Process optimization