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Extraction and Fractionation of Human Gastric Mucins from Gastric Juice
Rei Kawashima1, Daigo Tsubokawa2, Katsunori Iijima3
1Department of Biochemistry, Kitasato University School of Allied Health Science, Sagamihara, Japan.
Abstract:
Mucin, a major component of the mucus, is considered to be one of the principal factors in the physiological defense mechanism of the gastrointestinal mucosa. Measuring the mucin content of human gastric mucus is a useful tool for the assessment of Helicobacter pylori (H. pylori) eradication or the involvement of mucus secretion in various gastroduodenal diseases. Here, we describe a methodology for the isolation of the mucin fraction from human gastric juice and the quantification of mucin.
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