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Updated: Jul 3, 2025
Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution
Published on: September 21, 2012
Supported Immunohistochemical Staining of Mucins
1Department of Pathology, Field of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. east@m2.kufm.kagoshima-u.ac.jp.
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Immunohistochemistry (IHC) staining is the most common method to detect the distribution and localization of biomarkers in different parts of a tissue. Antibodies for tandem repeat peptide of mucins are very popular, but antibodies for glycosylation or others are also used. IHC for mucin is the same protocol as IHC for others. This description includes IHC according to ABC method for processed formalin-fixed paraffin-embedded (FFPE) tissues. Protocol of in situ hybridization is also shown.
