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Updated: Jul 3, 2025

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
Preparation of O-Glycans from Mucins Using Hydrazine Treatment
Yukinobu Goso1, Makoto Kurihara1
1Department of Applied Bioscience, Kanagawa Institute of Technology, Atsugi, Japan.
Abstract:
Mucin glycomic analysis is crucial owing to the participation of mucin O-glycans in several biological functions. Liquid chromatographic analysis of fluorescently labeled glycans is an effective tool for glycomic analysis. The first step of this analysis involves the release of O-glycans from mucins. As no enzyme is known to release all glycans, chemical methods are required for the process; therefore, hydrazine treatment is a commonly used chemical method. It enables the release of O-glycans from mucin while preserving the aldehyde group at the reducing end. This ensures that the reducing end can be modified using fluorescent reagents. However, it is also accompanied by the degradation of the glycans through a process called "peeling." Here, we describe a method for releasing glycans from mucins using hydrazine treatment with minimal "peeling."
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