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Updated: Jul 3, 2025

Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Structural Elucidation of Sialylated O-Glycan Alditols Obtained from Mucins by Mass Spectrometry
Daigo Tsubokawa1, Rei Kawashima2, Takafumi Ichikawa3
1Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Japan.
Abstract:
Acidic O-glycans having sialic acid and/or sulfate residue are abundantly expressed in intestinal mucins. However, structural elucidation of acidic O-glycans is a laborious and time-consuming task due to their large structural variations. Here, we describe a methodology of structural elucidation for sialylated O-glycan alditols from intestinal mucins using tandem mass spectroscopy. Methylesterification and mild periodate oxidation of sialylated O-glycan alditols assist mass analysis. This description includes the purification process of O-glycan alditols for structural analysis.
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