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Updated: Jul 3, 2025
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
Published on: January 22, 2014
RNA sequencing reveals differential long noncoding RNA expression profiles in bacterial and viral meningitis in
Xin Li1,2, Suzhen Sun2, Huifeng Zhang3,4
1Department of Pediatrics, The Second Hospital of Hebei Medical University, Hebei Medical University, No. 215 West Heping Street, Shijiazhuang, Hebei, 050000, China.
Background:
We aimed to investigate the involvement of long non-coding RNA (lncRNA) in bacterial and viral meningitis in children.
Methods:
The peripheral blood of five bacterial meningitis patients, five viral meningitis samples, and five healthy individuals were collected for RNA sequencing. Then, the differentially expressed lncRNA and mRNA were detected in bacterial meningitis vs. controls, viral meningitis vs. healthy samples, and bacterial vs. viral meningitis patients. Besides, co-expression and the competing endogenous RNA (ceRNA) networks were constructed. Receiver operating characteristic curve (ROC) analysis was performed.
Results:
Compared with the control group, 2 lncRNAs and 32 mRNAs were identified in bacterial meningitis patients, and 115 lncRNAs and 54 mRNAs were detected in viral meningitis. Compared with bacterial meningitis, 165 lncRNAs and 765 mRNAs were identified in viral meningitis. 2 lncRNAs and 31 mRNAs were specific to bacterial meningitis, and 115 lncRNAs and 53 mRNAs were specific to viral meningitis. The function enrichment results indicated that these mRNAs were involved in innate immune response, inflammatory response, and immune system process. A total of 8 and 1401 co-expression relationships were respectively found in bacterial and viral meningitis groups. The ceRNA networks contained 1 lncRNA-mRNA pair and 4 miRNA-mRNA pairs in viral meningitis group. GPR68 and KIF5C, identified in bacterial meningitis co-expression analysis, had an area under the curve (AUC) of 1.00, while the AUC of OR52K2 and CCR5 is 0.883 and 0.698, respectively.
Conclusions:
Our research is the first to profile the lncRNAs in bacterial and viral meningitis in children and may provide new insight into understanding meningitis regulatory mechanisms.
Insights
This study reveals long non-coding RNAs (lncRNAs) are involved in pediatric bacterial and viral meningitis. Differentially expressed lncRNAs and messenger RNAs (mRNAs) were identified, offering insights into meningitis pathogenesis.
Area of Science:
- Molecular Biology
- Genomics
- Pediatric Infectious Diseases
Background:
- Meningitis, a serious central nervous system infection, can be caused by bacteria or viruses.
- The role of long non-coding RNAs (lncRNAs) in pediatric meningitis remains largely unexplored.
Purpose of the Study:
- To investigate the involvement and differential expression of lncRNAs in children with bacterial and viral meningitis.
- To identify potential regulatory networks and biomarkers associated with meningitis.
Main Methods:
- RNA sequencing was performed on peripheral blood samples from children with bacterial meningitis, viral meningitis, and healthy controls.
- Differential expression analysis of lncRNAs and mRNAs was conducted.
- Co-expression and competing endogenous RNA (ceRNA) networks were constructed.
- Receiver operating characteristic (ROC) curve analysis was used to evaluate potential biomarkers.
Main Results:
- Significant numbers of differentially expressed lncRNAs and mRNAs were identified in both bacterial and viral meningitis compared to controls, and between the two types of meningitis.
- Functional enrichment analysis indicated involvement in immune response pathways.
- Specific lncRNAs and mRNAs were identified with potential diagnostic value, including GPR68, KIF5C, OR52K2, and CCR5.
Conclusions:
- This is the first study to profile lncRNAs in pediatric bacterial and viral meningitis.
- The findings provide novel insights into the regulatory mechanisms of meningitis and identify potential diagnostic biomarkers.
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