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MicroRNA-150 Deletion Reduces the Occurrence and Severity of Rheumatoid Arthritis by Inhibiting IL-17
1Department of ICU, The Affiliated Hospital of North China University of Science and Technology, Tangshan, China.
Background:
Understanding the effects of epigenetic factors on the pathogenesis of rheumatoid arthritis (RA) is important for the early diagnosis and therapeutic intervention of this disease. MicroRNA-150 (miR-150) exerts an important influence on the development and function of lymphocytes. However, the role of miR-150 in the pathogenesis of RA remains unclear.
Objective:
To explore the role of miR-150 in the pathogenesis of RA and the related immune mechanism.
Methods:
In this study, we used miR-150 knock-out (miR-150KO) and created animal models of RA. Flow cytometry, immunohistochemistry, and real-time RT-PCR were employed to assess the frequency of T cell subsets and cytokines expression.
Results:
Compared to wild-type (WT) mice, the onset of RA was postponed and the incidence of RA was reduced in miR-150KO mice. The expression of IL-4 and IFN-γ significantly increased while the expression of IL-17 decreased significantly in NKT and CD4+ T cells of KO mice compared to that of WT mice after RA induction. In addition, the expression of IL-4 and IFN-γ increased while the expression of IL-17 decreased significantly in the joint tissues of KO mice compared to that of WT mice. Furthermore, the mRNA expression of TNF-α and IL-17 decreased significantly in the synovial fluid cells of KO mice compared to that of the WT mice after RA induction.
Conclusion:
MiR-150 deficiency decreases the expression of IL-17 in T cells and joint tissues, and alleviates the occurrence and progression of RA in mice.
Insights
MicroRNA-150 deficiency alleviates rheumatoid arthritis (RA) in mice by reducing IL-17 expression in T cells and joint tissues, suggesting a therapeutic target for RA.
Area of Science:
- Immunology
- Epigenetics
- Rheumatology
Background:
- Epigenetic factors are crucial for rheumatoid arthritis (RA) pathogenesis, impacting early diagnosis and treatment.
- MicroRNA-150 (miR-150) influences lymphocyte development and function, but its specific role in RA is not fully understood.
Purpose of the Study:
- To investigate the role of miR-150 in rheumatoid arthritis (RA) pathogenesis.
- To elucidate the underlying immune mechanisms involving miR-150 in RA.
Main Methods:
- Utilized miR-150 knock-out (miR-150KO) mouse models to study RA.
- Employed flow cytometry, immunohistochemistry, and real-time RT-PCR to analyze T cell subsets and cytokine expression.
Main Results:
- miR-150KO mice exhibited delayed RA onset and reduced incidence compared to wild-type (WT) mice.
- KO mice showed increased IL-4 and IFN-γ, and decreased IL-17 in T cells and joint tissues.
- Reduced mRNA expression of TNF-α and IL-17 was observed in synovial fluid cells of KO mice.
Conclusions:
- MiR-150 deficiency mitigates RA development and progression in mice.
- Reduced IL-17 expression in T cells and joint tissues is a key mechanism by which miR-150 deficiency alleviates RA.
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