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Updated: Jul 3, 2025

Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro
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Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro

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SCUBE3 Exerts a Tumor-Promoting Effect in Tongue Squamous Cell Carcinoma by Promoting CEBPA Binding to the CCL2

Minhui Zhu1, Yi Ma1, Wei Wang1

  • 1Department of Otorhinolaryngology-Head and Neck Surgery, Changhai Hospital, Naval Medical University, Shanghai, P.R. China.

Molecular Cancer Research : MCR
|February 13, 2024
PubMed

Insights

Signal peptide-CUB-EGF domain-containing protein 3 (SCUBE3) promotes tongue squamous cell carcinoma (TSCC) growth. Targeting the SCUBE3/CEBPA/CCL2/STAT3 pathway may offer new therapeutic strategies for oral cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Tongue squamous cell carcinoma (TSCC) is a major oral cancer subtype with limited treatment options.
  • Signal peptide-CUB-EGF domain-containing protein 3 (SCUBE3) is implicated in various cancers, but its role in TSCC is unclear.

Purpose of the Study:

  • To investigate the expression and function of SCUBE3 in TSCC.
  • To elucidate the underlying molecular mechanisms of SCUBE3 in TSCC progression.

Main Methods:

  • Analysis of SCUBE3 expression in TSCC tissues.
  • In vitro experiments involving SCUBE3 knockdown and CCL2 overexpression in TSCC cells.
  • In vivo tumorigenicity assays.
  • Western blotting and promoter binding assays to explore molecular pathways.

Main Results:

  • SCUBE3 is highly expressed in TSCC, correlating with advanced tumor and T stages.
  • SCUBE3 silencing inhibited TSCC cell proliferation, migration, invasion, and in vivo tumor growth, while inducing apoptosis and G2-phase cell cycle arrest.
  • SCUBE3 promotes TSCC by facilitating CEBPA binding to the CCL2 promoter, activating the CCL2/STAT3 signaling axis.

Conclusions:

  • SCUBE3 acts as a tumor promoter in TSCC through the CEBPA/CCL2/STAT3 pathway.
  • SCUBE3 represents a potential therapeutic target for TSCC, offering new avenues for oral cancer treatment.

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