Integrated physiological, metabolomic, and transcriptomic analyses elucidate the regulation mechanisms of lignin synthesis under osmotic stress in alfalfa leaf (Medicago sativa L.)
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View abstract on PubMed
Summary
This summary is machine-generated.Osmotic stress increases lignin in alfalfa leaves, enhancing plant resistance. This study identifies key genes and signaling pathways involved in lignin biosynthesis, aiding the development of stress-resilient alfalfa varieties.
Area Of Science
- Plant Biology
- Agricultural Science
- Biochemistry
Background
- Alfalfa is a vital forage crop, but its yield and quality are threatened by environmental stressors.
- Lignin, a cell wall component, confers resistance to abiotic stress, yet its response to osmotic stress in alfalfa leaves is not well understood.
Purpose Of The Study
- To investigate the impact of osmotic stress on lignin accumulation in alfalfa leaves.
- To analyze changes in lignin synthesis intermediates and related gene expression under osmotic stress.
Main Methods
- Transcriptome and metabolome analyses were integrated to identify key genes and metabolites.
- Enzyme activities and gene expression in the phenylpropanoid pathway were examined.
Main Results
- Osmotic stress led to increased lignin accumulation in alfalfa leaves.
- Alterations in core enzyme activities and gene expression within the phenylpropanoid pathway were observed.
- Five hub genes (CSE, CCR, CADa, CADb, POD) and thirty edge genes were identified.
- Abscisic acid (ABA) and ethylene signaling showed contradictory regulation of lignin biosynthesis.
Conclusions
- Osmotic stress significantly influences lignin biosynthesis in alfalfa leaves.
- Key genes and signaling pathways regulating lignin accumulation under stress were identified.
- These findings provide a theoretical basis for breeding improved, stress-resistant alfalfa varieties.
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