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Updated: Jul 3, 2025
PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP
Published on: April 9, 2013
Antimicrobial effects of clindamycin-loaded platelet-rich fibrin (PRF)
Anton Straub1, Maximilian Stapf2, Chiara Utz3
1Department of Oral and Maxillofacial Plastic Surgery, University Hospital Würzburg, Pleicherwall 2, 97070, Würzburg, Germany. Straub_A@ukw.de.
Objectives:
Recent research has demonstrated that platelet-rich fibrin (PRF) is an appropriate carrier for ampicillin/sulbactam. The aim of the study was to investigate whether PRF is also a suitable bio-carrier for clindamycin (CLI).
Methods:
PRF membranes were produced from 36 patients receiving intravenous therapy with CLI (e.g. due to the diagnosis of an osteonecrosis of the jaw or infections). Concentrations of CLI in PRF membranes were measured with liquid chromatography-tandem mass spectrometry, and the antimicrobial effects were investigated in vitro in agar diffusion tests with fresh PRF and PRF stored for 24 h. Storage was performed in an incubator at 36 °C to simulate the in-vivo situation.
Results:
The mean concentration of CLI in plasma was 1.0 ± 0.3 μg/100 mg plasma; in resulting PRF membranes 0.7 ± 0.4 μg/100 mg PRF. Agar diffusion tests were performed with Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus mitis, Porphyromonas gingivalis, and Fusobacterium nucleatum. Mean inhibition zones, in mm, for fresh PRF were 17.3, 12.2, 18.8, 17.1, 25.8 and 18.1, 12.7, 19.2, 17.3, and 26.3 for stored PRF, respectively.
Conclusion:
The results demonstrate that PRF is a suitable bio-carrier for CLI when administered systemically to patients. The concentration in PRF generated from patients after infusion of 600 mg CLI dose suffices to target clinically relevant bacteria.
Clinical Relevance:
Using PRF as a carrier for local antibiotic application can prevent infections in oral and maxillofacial surgery. Within the study limitations, the findings could expand the scope of PRF application by adding CLI as a new antibiotic to the spectrum of PRF therapy.
Insights
Platelet-rich fibrin (PRF) effectively carries clindamycin (CLI), demonstrating its suitability as a bio-carrier. This finding supports PRF
Area of Science:
- Biomaterials Science
- Infectious Diseases
- Oral and Maxillofacial Surgery
Background:
- Platelet-rich fibrin (PRF) has shown potential as a carrier for antibiotics like ampicillin/sulbactam.
- Clindamycin (CLI) is a commonly used antibiotic for various infections.
Purpose of the Study:
- To evaluate if PRF can serve as a suitable bio-carrier for clindamycin (CLI).
- To assess the concentration and antimicrobial efficacy of clindamycin within PRF membranes.
Main Methods:
- PRF membranes were prepared from 36 patients receiving intravenous clindamycin.
- Clindamycin concentrations in PRF were quantified using liquid chromatography-tandem mass spectrometry.
- In vitro antimicrobial activity was tested against relevant bacteria using agar diffusion assays.
Main Results:
- Mean clindamycin concentration in PRF membranes was 0.7 ± 0.4 μg/100 mg PRF.
- Antimicrobial activity was observed against Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus mitis, Porphyromonas gingivalis, and Fusobacterium nucleatum.
- Inhibition zones indicated effective antimicrobial action for both fresh and 24-hour stored PRF.
Conclusions:
- Platelet-rich fibrin is a suitable bio-carrier for systemically administered clindamycin.
- The clindamycin concentration in PRF is sufficient to target clinically relevant bacteria.
- PRF holds potential for expanded applications in local antibiotic delivery for infection prevention in oral and maxillofacial surgery.
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