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Updated: Aug 8, 2026

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
Published on: May 2, 2018
Histological and Cytological Techniques to Study Perinatal Mouse Ovaries and Oocytes
Nikoleta Nikou1,2, Maria López Panadés1,2, Ignasi Roig3,4
1Genome Integrity and Instability Group, Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Barcelona, Spain.
Abstract:
The regulation of female fertility in mammals depends on critical processes during oocyte development and maturation. Therefore, it is crucial to use specific approaches when studying mammalian female fertility to preserve ovary and oocyte structures effectively. The methods of collecting and culturing ovaries and oocytes play an essential role in the study of mammalian follicle development and oocyte quality. This chapter presents a collection of protocols that focus on various methods for studying mammalian ovaries and oocytes, providing researchers with a variety of approaches to choose from.

