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Updated: Jul 3, 2025

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
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High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

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Predicting the DNA binding specificity of transcription factor mutants using family-level biophysically interpretable

Shaoxun Liu1, Pilar Gomez-Alcala1, Christ Leemans1

  • 1Department of Biological Sciences, Columbia University, New York, NY, USA.

Biorxiv : the Preprint Server for Biology
|February 14, 2024
PubMed
Summary

This study introduces a novel method to predict how mutations in transcription factors (TFs) alter their DNA binding preferences. This approach accurately forecasts changes in binding free energy for TF mutants, aiding disease mutation impact analysis.

Keywords:
DNA binding specificitybasic helix-loop-helix (bHLH)biophysically interpretable machine learningfunctional impact of missense mutationshigh-throughput SELEXhomeodomainprotein binding microarray (PBM)transcription factors (TFs)

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