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Genomic DNA in Prokaryotes

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...

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Updated: Jul 3, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

scMicrobe PTA: Near Complete Genomes from Single Bacterial Cells.

Robert M Bowers¹, Veronica Gonzalez-Pena², Kartika Wardhani²

¹DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.

Biorxiv : the Preprint Server for Biology

|February 14, 2024

Summary

This summary is machine-generated.

Primary template amplification (PTA) generates nearly complete microbial genomes from single cells. This novel technique significantly improves genome recovery from diverse environments compared to standard methods.

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Last Updated: Jul 3, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

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Published on: August 20, 2021

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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Area of Science:

Microbiology
Genomics
Molecular Biology

Background:

Single-cell amplification techniques are crucial for studying microbial communities.
Existing methods often yield incomplete microbial genomes, limiting downstream analysis.
Obtaining high-quality genomes from environmental samples remains a challenge.

Approach:

Introduced primary template amplification (PTA), a novel single-cell amplification technique.
Applied PTA to bacterial isolates and diverse aquatic and soil microbiomes.
Compared PTA-derived genome completeness with standard amplification methods.

Key Points:

PTA generated nearly complete genomes from three bacterial isolates.
PTA achieved a median genome completeness of 81% for diverse environmental samples.
Standard amplification methods yielded genomes with <30% completeness.
PTA-derived genomes contained more associated viruses and biosynthetic gene clusters.

Conclusions:

Primary template amplification is a highly effective method for recovering near-complete microbial genomes from single cells.
PTA significantly advances the ability to perform in-depth genomic analysis of unculturable bacteria and complex microbial communities.
This technique has broad implications for microbial ecology, synthetic biology, and the discovery of novel bioactive compounds.