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Multiplexed Short-wave Infrared Imaging Highlights Anatomical Structures in Mice.

Xingjian Zhong1,2, Amish Patel1,2, Yidan Sun2

  • 1Department of Biomedical Engineering, Boston University, Boston, MA, 02215, USA.

Biorxiv : the Preprint Server for Biology
|February 14, 2024
PubMed
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Researchers developed advanced short-wave infrared quantum dots for simultaneous three-color in vivo imaging in mice. This breakthrough enables deeper tissue penetration and precise visualization of biological systems for preclinical research.

Area of Science:

  • Biomedical Imaging
  • Nanotechnology
  • Optical Engineering

Background:

  • Multiplexed fluorescence imaging is limited in vivo by light scattering and attenuation at visible and traditional near-infrared (NIR-I) wavelengths.
  • Short-wave infrared (SWIR, 1000–1700 nm, also known as NIR-II) imaging offers deeper tissue penetration and reduced background autofluorescence for preclinical applications.
  • Current SWIR imaging is typically limited to two-color schemes, restricting multiplexing capabilities.

Approach:

  • Engineered three distinct, high quantum yield lead sulfide/cadmium sulfide (PbS/CdS) core/shell quantum dots (QDs) with tunable SWIR emissions (1100–1550 nm).
  • Utilized these QDs for simultaneous three-color fluorescence imaging in mouse models.
  • Demonstrated non-invasive tracking of lymphatic drainage and visualization of lymphatic and vasculature networks.

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Key Points:

  • Achieved simultaneous three-color SWIR imaging in mice using novel PbS/CdS core/shell QDs.
  • Successfully visualized detailed lymphatic vessel networks over a 2-hour period.
  • Distinguished spatially overlapping lymphatic and vasculature networks using multiplexed SWIR imaging.

Conclusions:

  • Developed optimized SWIR QDs for next-generation multiplexed preclinical imaging, surpassing previous dual-labeling limitations.
  • The ability to discriminate multiple fluorescent labels via NIR-I excitation and SWIR detection opens new avenues for disease progression, drug biodistribution, and cell trafficking studies.
  • This technique enhances non-invasive anatomical imaging in small animal models.