Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

56.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.4K
Alternative RNA Splicing02:18

Alternative RNA Splicing

21.2K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.2K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.2K
5.2K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

2.7K
2.7K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K
RNA Editing02:23

RNA Editing

9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeted mutagenesis and base editing using engineered <i>Brevibacillus laterosporus</i> Cas9 with expanded target scope in rice.

Plant biotechnology (Tokyo, Japan)·2026
Same author

Single-Molecule Characterization of CRISPR-Cas12a for Amplification-Free Genetic Testing.

Analytical chemistry·2026
Same author

Laparoscopic Splenectomy for Sclerosing Angiomatoid Nodular Transformation of the Spleen after a Whipple Procedure: A Case Report.

Surgical case reports·2026
Same author

Function through shape: An overview of DNA G-quadruplexes in transcriptional regulation.

Current opinion in chemical biology·2026
Same author

Structural mechanism of SAM-AMP and SAM-AMP<sub>2</sub> synthesis by the type III-D2 CRISPR effector complex.

Nature communications·2026
Same author

Structure and engineering of the large serine recombinase Bxb1 for gene integration.

Molecular cell·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jul 3, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.2K

Programmable RNA writing with trans-splicing.

Cian Schmitt-Ulms1, Alisan Kayabolen1, Marcos Manero-Carranza1

  • 1McGovern Institute for Brain Research at MIT, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Biorxiv : the Preprint Server for Biology
|February 14, 2024
PubMed
Summary
This summary is machine-generated.

Introducing Programmable RNA Editing & Cleavage for Insertion, Substitution, and Erasure (PRECISE), a novel RNA editing tool. PRECISE enables precise, large RNA insertions, deletions, and substitutions into the transcriptome without permanent off-target effects.

More Related Videos

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.0K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.7K

Related Experiment Videos

Last Updated: Jul 3, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.2K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.0K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.7K

Area of Science:

  • Molecular Biology
  • Genetic Engineering
  • RNA Therapeutics

Background:

  • RNA editing offers transient transcriptome modification without permanent off-target effects, but arbitrary edits remain challenging.
  • Current RNA editing tools have limitations in scope and efficiency for complex genetic modifications.

Approach:

  • Developed Programmable RNA Editing & Cleavage for Insertion, Substitution, and Erasure (PRECISE), a method utilizing Cas7-11 cleavage to enhance RNA trans-splicing efficiency.
  • PRECISE introduces exogenous templates for precise exon replacement or insertion into pre-mRNAs, achieving high editing rates (5-50% endogenous, 85% reporter).

Key Points:

  • Demonstrated PRECISE editing across 11 endogenous transcripts, enabling over 50 edit types, including all base transitions/transversions, and insertions/deletions up to 1,863 nucleotides.
  • Successfully edited MECP2 for Rett Syndrome, SHANK3 for Autism, and HTT for Huntington's disease, showcasing therapeutic potential.
  • Whole transcriptome sequencing confirmed high precision and lack of off-target trans-splicing activity.

Conclusions:

  • PRECISE editing broadens genetic editing capabilities, offering a versatile alternative to existing tools like prime editing.
  • Achieved protein-free, high-efficiency trans-splicing via payload engineering and ribozymes, with successful AAV delivery for Huntington's disease models.
  • PRECISE editing functions in non-dividing cells and addresses a wider spectrum of genetic diseases, including those not treatable by current RNA base editors.