[Development of a carbamazepine determination method based on high-performance liquid chromatography with diode...

Area of Science:

• Analytical Chemistry
• Forensic Toxicology
• Pharmacology

Context:

• Carbamazepine is a vital medication for treating epilepsy, neuropathic pain, and bipolar disorder.
• Adverse drug reactions and potential overdose risks necessitate precise therapeutic drug monitoring.
• Forensic chemical analysis requires reliable methods for carbamazepine quantification in biological samples.

Purpose:

• To develop and validate a robust method for carbamazepine quantification.
• To ensure accurate measurement of carbamazepine levels in forensic investigations.
• To establish stable analytical conditions for reproducible results.

Summary:

• A high-performance liquid chromatography method with diode array detection was established for carbamazepine quantitation.
• The method demonstrated stability across varied analytical conditions, including eluent composition, flow rate, temperature, and pH.
• Excellent reproducibility was confirmed, with relative standard deviations for retention time and peak area below 0.5%.

Impact:

• Provides a reliable analytical tool for forensic toxicologists.
• Enhances patient safety through accurate therapeutic drug monitoring.
• Contributes to the accurate assessment of carbamazepine levels in legal and clinical contexts.