Complete genome sequence of Citrobacter braakii ASE1 generated by PacBio sequencing
Thao D Tran1, SangIn Lee1, Robert Hnasko2
1Foodborne Toxin Detection and Prevention Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California, USA.
Microbiology Resource Announcements
|February 14, 2024
Summary
We sequenced the complete genome of Citrobacter braakii ASE1, a bacterium found in California lettuce farm soil. This provides valuable genomic data for this important agricultural soil microbe.
Area of Science:
- Microbiology
- Genomics
- Agricultural Science
Background:
- Citrobacter braakii is a species of Gram-negative bacteria.
- Soil microorganisms play crucial roles in agricultural ecosystems.
Purpose of the Study:
- To present the complete genome sequence of Citrobacter braakii ASE1.
- To provide a genomic resource for agricultural and environmental microbiology.
Main Methods:
- Whole genome sequencing using the PacBio Sequel II platform.
- Bioinformatic analysis of the resulting sequence data.
Main Results:
- The complete genome sequence of Citrobacter braakii ASE1 was successfully generated.
- The genome comprises a single circular chromosome of 5,021,820 base pairs.
- The GC content of the genome was determined to be 52.2%.
Conclusions:
- The availability of the complete genome sequence facilitates further research into Citrobacter braakii.
- This genomic data can aid in understanding the bacterium's role in agricultural soil environments.
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