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Alterations of gut microbiota in infants with biliary atresia identified by 16S rRNA-sequencing

Fei Liu1, Ru Wei2, Xiaogang Xu1

  • 1Department of Pediatric Surgery, Guangdong Provincial Clinical Research Center for Child Health, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 9 Jinsui Road, Guangzhou, Guangdong, 510623, China.

BMC Pediatrics
|February 14, 2024
PubMed

Insights

Gut microbiota differs in infants with biliary atresia (BA). The Klebsiella/Bifidobacterium ratio may serve as a biomarker for identifying BA, aiding in treatment strategies.

Area of Science:

  • Neonatal diseases
  • Gastroenterology
  • Microbiome research

Background:

  • Biliary atresia (BA) is a severe neonatal condition causing bile duct inflammation and liver fibrosis.
  • Understanding the gut microbiome in BA infants is crucial for identifying biomarkers.
  • This study investigates the gut microbiota's role in biliary atresia.

Purpose of the Study:

  • To explore the relationship between gut microbiota composition and biliary atresia.
  • To identify potential non-invasive biomarkers for BA diagnosis.

Main Methods:

  • 16S ribosomal RNA (rRNA) gene sequencing was used to analyze gut microbiota.
  • Compared microbiota in 31 infants with BA and 20 healthy controls.

Main Results:

  • Significant differences in gut microbiota composition were observed between BA and control groups (P < 0.05).
  • The Klebsiella/Bifidobacterium abundance ratio showed high potential for BA identification (P < 0.01).
  • Differentially abundant bacteria were involved in lipid and vitamin metabolism.

Conclusions:

  • Gut microbiota alterations are linked to biliary atresia.
  • The Klebsiella/Bifidobacterium ratio may serve as a non-invasive biomarker for BA.
  • Findings can inform treatments targeting microbiota dysbiosis in BA.
Abstract

Keywords:
16S rRNA-sequencingBiliary atresiaBiomarkerGut microbiotaInfant

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