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Differences in enteric neuronal density in the NSE-Noggin mouse model across institutes
Simone L Schonkeren1, Meike S Thijssen1,2, Musa Idris1,3
1Department of Pathology, GROW-Research Institute for Oncology and Reproduction, Maastricht University Medical Center, Maastricht, The Netherlands.
Scientific Reports
|February 15, 2024
Summary
The NSE-Noggin mouse model, intended to study the enteric nervous system (ENS), did not show increased neuronal density in the colon. Further investigation is needed to understand why this model failed to exhibit the expected hyperganglionic phenotype.
Area of Science:
- Neuroscience
- Gastroenterology
- Developmental Biology
Background:
- The enteric nervous system (ENS) is crucial for gut function.
- Studying the ENS requires specialized animal models.
- The NSE-Noggin mouse was proposed as a model for hyperinnervation.
Purpose of the Study:
- To validate the NSE-Noggin mouse model for studying enteric nervous system hyperinnervation.
- To investigate enteric neuronal density and subtype markers in NSE-Noggin mice.
Main Methods:
- Phenotyping, genotyping, and DNA sequencing of NSE-Noggin mice.
- RNA and protein expression analysis (Noggin, EGFP).
- Quantification of enteric neuron density and subtype markers in colonic myenteric plexus.
Main Results:
- NSE-Noggin mice confirmed transgene presence and Noggin expression.
- No significant difference in total enteric neuron density was observed compared to wild-type mice.
- Proportions of specific neuronal subtypes (calbindin, calretinin, serotonin) remained unchanged.
Conclusions:
- The NSE-Noggin mouse model did not exhibit the expected hyperganglionic phenotype in our study.
- Discrepancies may be due to study design, microbiota, or environmental factors.
- Further research is required to elucidate the reasons for the lack of hyperinnervation.
