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Updated: Jul 3, 2025

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
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Alteration of DNA methyltransferases by eribulin elicits broad DNA methylation changes with potential therapeutic

Meisam Bagheri1,2, Min Kyung Lee3, Kristen E Muller2,4

  • 1Department of Molecular & Systems Biology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, USA.

Epigenomics
|February 15, 2024
PubMed

Insights

Eribulin alters DNA methylation and epigenetic modifiers in triple-negative breast cancer (TNBC) cells, offering new therapeutic strategies. This research highlights eribulin

Area of Science:

  • Oncology
  • Pharmacology
  • Epigenetics

Background:

  • Triple-negative breast cancer (TNBC) is an aggressive subtype with limited therapeutic options.
  • Eribulin, a chemotherapy agent, exhibits potential unique mechanisms of action through epigenetic modifications.

Purpose of the Study:

  • To investigate the epigenetic effects of eribulin in TNBC.
  • To identify potential biomarkers for predicting treatment response to eribulin.

Main Methods:

  • Treatment of MDA-MB 231 cells with eribulin and paclitaxel.
  • Immunohistochemical analysis of patient samples from eribulin-treated and standard-of-care groups.
  • Assessment of DNA methylation and expression of epigenetic modifiers (DNMT1, TET1, DNMT3A/B).

Main Results:

  • Eribulin induced significant DNA methylation changes in drug-tolerant persister TNBC cells.
  • Eribulin altered the expression of epigenetic modifiers both in vitro and in primary TNBC tumors.
  • Distinct epigenetic profiles were observed between eribulin-treated and standard-of-care groups.

Conclusions:

  • Eribulin's mechanism of action involves significant epigenetic alterations in TNBC.
  • Epigenetic modifiers may serve as predictive biomarkers for eribulin efficacy in TNBC treatment.
Keywords:
DNA methylationbreast cancerchemotherapyepigeneticepithelial-to-mesenchymal transition

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