A pan-cancer characterization of immune-related NFIL3 identifies potential predictive biomarker
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View abstract on PubMed
Summary
This summary is machine-generated.Nuclear factor interleukin 3 (NFIL3) is downregulated in cancers and may serve as a prognostic biomarker. This study explores NFIL3
Area Of Science
- Oncology
- Immunology
- Molecular Biology
Background
- Nuclear factor interleukin 3 (NFIL3) is known for regulating circadian rhythms and immune responses.
- Its role in human cancers remains under-explored.
Purpose Of The Study
- To investigate the expression, prognostic value, and potential functions of NFIL3 across various human cancers.
- To explore the association of NFIL3 with tumor microenvironment factors and the p53 signaling pathway.
Main Methods
- Utilized TCGA, TARGET, and GTEx datasets for pan-cancer analysis of NFIL3 expression.
- Assessed prognostic implications using GEPIA, KM Plotter, and PrognoScan.
- Investigated correlations with genomic heterogeneity, tumor mutational burden (TMB), microsatellite instability (MSI), tumor purity, neoantigens, immune cell infiltration, and immune checkpoint genes.
- Validated findings in ovarian cancer cell lines using proliferation, migration, and molecular assays (qRT-PCR, Western blot, Co-IP).
Main Results
- NFIL3 expression was generally decreased in cancerous tissues compared to normal tissues.
- NFIL3 expression correlated significantly with prognosis, immune cell infiltration, immune checkpoint genes, TMB, MSI, tumor purity, and neoantigens.
- Experimental data suggested NFIL3 influences ovarian cancer cell migration and proliferation.
- A strong association between NFIL3 and the p53 signaling pathway was identified and validated.
Conclusions
- NFIL3 may function as a potential pan-cancer biomarker for prognosis and immunotherapy.
- NFIL3's role in cancer progression and its interaction with the p53 pathway warrant further investigation.
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