Comparative effect of anthocyanin on proliferation and migration of human gingival fibroblasts in the absence or presence of nicotine

Sarina Azimian ¹, Maryam Torshabi ², Zeinab Rezaei Esfahrood ³

⁰Department of Periodontics, Shahid Beheshti Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Journal of Advanced Periodontology & Implant Dentistry +

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February 15, 2024

View abstract on PubMed

Summary

Cyanidin chloride (CC) shows promise in mitigating nicotine

Area Of Science

Oral biology
Periodontology
Pharmacology

Background

Oral fibroblast dysfunction contributes to periodontal diseases.
Nicotine, an oral tissue irritant, impedes wound healing.
Anthocyanins, like cyanidin chloride (CC), may combat smoking-related periodontal issues.

Purpose Of The Study

To investigate the effects of cyanidin chloride (CC) on human gingival fibroblast (HGF) viability, proliferation, and migration.
To assess CC's protective potential against nicotine-induced cellular damage in an in vitro setting.

Main Methods

Human gingival fibroblasts (HGFs) were exposed to varying concentrations of nicotine and cyanidin chloride (CC).
Cell viability and proliferation were quantified using the MTT assay.
Cell migration was evaluated using the scratch test in the presence of nicotine.

Main Results

Cyanidin chloride (CC) up to 100 μM showed no cytotoxicity to HGFs.
Nicotine (3-5 mM) significantly reduced HGF viability and proliferation.
CC partially counteracted nicotine's negative impact on HGF migration.

Conclusions

Cyanidin chloride (CC) demonstrated a significant protective effect on human gingival fibroblast (HGF) migration despite nicotine exposure.
While CC did not fully restore viability and proliferation, its positive impact on migration warrants further investigation for periodontal disease treatment.

Keywords:

Anthocyanins Fibroblasts Migration Nicotine Proliferation