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A Novel Multi-compartment Rotating Bioreactor for Improving ADSC-Spheroid Formation and its Application in Neurogenic

Peng Wang¹, Yang Liu², Xiao-Feng Duan¹

¹Department of Urology, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, 121000, China.

Current Stem Cell Research & Therapy

|February 15, 2024

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A novel bioreactor efficiently produces adipose-derived stem cell (ADSC) microspheres for treating erectile dysfunction (ED). This method significantly improved erectile function in a rat model, demonstrating its therapeutic potential.

Area of Science:

Biomedical Engineering
Regenerative Medicine
Urology

Background:

Erectile dysfunction (ED) poses a significant health challenge.
Adipose-derived stem cells (ADSCs) show promise for regenerative therapies.
Current methods for ADSC spheroid production lack efficiency and control.

Purpose of the Study:

To construct a multicompartment synchronous rotating bioreactor (MCSRB) for batch production of homogenized ADSC microspheres.
To evaluate the efficacy and safety of ADSC microspheres in treating neurogenic erectile dysfunction.

Main Methods:

Construction of an MCSRB using a centrifugal device and hinged trays.
Optimization of ADSC-spheroid formation by exploring density and rotational speed.
Establishment of a neurogenic ED rat model to assess treatment outcomes.

Keywords:

Centrifugation culture HUVECs.adipose-derived stem cells (ADSCs)cavernous nerve damage erectile dysfunction stem cell microspheres

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Main Results:

The MCSRB facilitated the production of ADSC microspheres with diameters of 90-130 μm.
Supernatant from 3D culture significantly enhanced cytokine expression in ADSCs and HUVEC migration.
ADSC microsphere treatment markedly improved erectile function and penile pathology in ED rats compared to traditional ADSC treatment (p < 0.01).

Conclusions:

The MCSRB enables efficient, batch, controlled, and homogenized production of ADSC stem cell microspheres.
The developed ADSC microspheres effectively improve erectile dysfunction in a neurogenic rat model.
This technology offers a promising therapeutic strategy for ED treatment.