NR5A2 promotes malignancy progression and mediates the effect of cisplatin in cutaneous squamous cell carcinoma
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View abstract on PubMed
Summary
This summary is machine-generated.Nuclear receptor subfamily five group A member two (NR5A2) promotes cutaneous squamous cell carcinoma (cSCC) progression. NR5A2 knockdown enhances cisplatin
Area Of Science
- Oncology
- Dermatology
- Molecular Biology
Background
- Nuclear receptor subfamily five group A member two (NR5A2) is implicated in various cancers.
- Its role in cutaneous squamous cell carcinoma (cSCC) remains unclear.
Purpose Of The Study
- To investigate the role of NR5A2 in cSCC proliferation.
- To determine if NR5A2 influences cisplatin's efficacy in cSCC.
Main Methods
- Bioinformatic analysis and immunohistochemistry to assess NR5A2 expression in cSCC tissues and cell lines.
- NR5A2 knockdown in cSCC cells to evaluate proliferation, cell cycle, apoptosis, and Wnt/β-catenin pathway.
- Assessing cisplatin's effects on NR5A2-knockdown cSCC cells using CCK-8, colony formation, and flow cytometry.
Main Results
- NR5A2 expression is elevated in cSCC tissues compared to healthy tissues.
- NR5A2 downregulation reduced cSCC malignancy and proliferation, while an agonist increased proliferation.
- NR5A2 knockdown decreased Wnt/β-catenin pathway activity, which was reversible.
- NR5A2 knockdown potentiated cisplatin's anti-proliferative and pro-apoptotic effects in cSCC cells.
Conclusions
- NR5A2 is crucial for cSCC progression, potentially via the Wnt/β-catenin pathway.
- Reducing NR5A2 enhances cisplatin's effectiveness in inhibiting proliferation and inducing apoptosis in cSCC.
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