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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Jul 3, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

Community recommendations on cryoEM data archiving and validation.

Gerard J Kleywegt¹, Paul D Adams², Sarah J Butcher³

¹EMBL-EBI, Cambridge, United Kingdom.

|February 15, 2024

Summary

This summary is machine-generated.

Experts met to define data standards for cryo-electron microscopy (cryoEM) structures, focusing on single-particle analysis. Recommendations were made for deposition, validation, and archiving to improve data quality and accessibility.

Keywords:

Electron Microscopy Data Bank Protein Data Bank bioinformatics cryogenic-specimen electron microscopy data archiving databases quality control single-particle cryoEM structure determination validation

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Last Updated: Jul 3, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

gP2S, an Information Management System for CryoEM Experiments

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Published on: June 10, 2021

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Area of Science:

Structural Biology
Biophysics
Data Science

Background:

Cryo-electron microscopy (cryoEM) is a powerful technique for determining the 3D structure of biological macromolecules.
Standardized data requirements are crucial for the reliable deposition, validation, and archiving of cryoEM structures.
Previous efforts have highlighted the need for community consensus on best practices in cryoEM data management.

Purpose of the Study:

To bring together experts to discuss and define data requirements for cryoEM structure deposition and validation.
To focus on single-particle analysis (SPA) data, a widely used cryoEM method.
To develop consensus recommendations for improving data quality, reproducibility, and accessibility in the field.

Main Methods:

Convened a workshop at EMBL-EBI with 47 experts from relevant fields (data processing, model building, refinement, validation, archiving).
Facilitated discussions on current challenges and future needs in cryoEM data management.
Documented consensus recommendations and identified areas for future methods development.

Main Results:

Generated a set of consensus recommendations for data deposition and validation in cryoEM, particularly for SPA.
Identified key challenges and areas requiring further development in cryoEM data processing and archiving.
Reported on the initial implementation status of some of the workshop's recommendations.

Conclusions:

The workshop successfully established a consensus on essential data requirements for cryoEM structures.
The recommendations aim to enhance the quality, integrity, and usability of structural data in the scientific community.
Continued efforts in methods development and implementation are necessary to fully realize the benefits of standardized data practices.